© Volker Lannert

Working Group Hansen

Methods

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© Volker Lannert

Synthesis of drug molecules

  • Medicinal chemistry
  • Microwave-assisted synthesis
  • Solid-phase synthesis
  • Combinatorial synthesis
  • Multi-component reactions
  • Compound classes: e.g. small molecules, peptides, peptidomimetics, PROTACs
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© Volker Lannert

Target Engagement Assays

  • Biochemical enzyme assays
  • Jump dilution assays
  • NanoBiT assays
  • CETSA
  • Western Blot
  • Microscale Thermophoresis (MST) assays
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© Volker Lannert

Cell biological characterization of drug molecules

  • Viability assays (MTT, WST-8, and Celltiter Glo)
  • Cell cycle analysis
  • Functional apoptosis assays (Annexin V/PI, Caspase 3/7)
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© Dr. Kathrin Tan

Molecular Modeling

  • Docking studies using Gold, MOE, RosettaLigand, and Autodock