Prof. Dr. Diana Imhof

University Bonn

Pharmaceutical Institute

Pharmaceutical Biochemistry and Bioanalytics

An der Immenburg 4

D-53121 Bonn


Telefon: 0228 73 5254
Fax: 0228 73 6829
E-Mail: dimhof [at]


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Bioanalytical techniques are a major focus of the "Protein Synthesis & Bioanalytics" core facility. A range of techniques that span from general bioanalytical applications to more specialized requirements are offered and undertaken.
  • HPLC analysis in analytical amounts
                        LC-10A HPLC system (Shimadzu)
                   LC-20A recycling HPLC system (Shimadzu)


For very complicated compounds the innovative recycling function of our LC-20A HPLC system can be used to produce results at higher resolutions.

  • LC-MS/MS
  • Amino acid analysis
LC-MS/MS can be used to confirm or analyze the molecular weight as well as the composition of various compounds. Subsequent application of ESI-MS/MS to tryptic digest is another standard method offered by the core facility. The core facility uses the LC-ESI micrOTOF-Q III mass spectrometer (Bruker Daltonics GmbH).

LC-ESI micrOTOF-Q III (Bruker Daltonics GmbH)
Amino acid analysis is regularly used to determine peptide and protein concentrations and composition. This is one of the most precise techniques that is still applied in food chemistry. The core facility uses an Eppendorf-Biotronik LC 3000 system (Laborservice Onken).

ASAEppendorf-Biotronik LC 3000 (Onken)
  • Additional techniques
Additional chromatographic and spectroscopic techniques can be requested to complete the analytical profile of the compounds of interest. Some of the techniques offered in this regard are listed below
  1. Thin layer chromatography
  2. UV/Vis-Spectroscopy (Cuvette or Microplate reader)
  Multiskan GO (Thermo Scientific)


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