Prof. Dr. Diana Imhof

Universität Bonn

Pharmazeutisches Institut

Pharmazeutische Biochemie und Bioanalytik

An der Immenburg 4

D-53121 Bonn


Telefon: 0228 73 5254
Fax: 0228 73 60223
E-Mail: dimhof [at]


Sie sind hier: Startseite Bereiche Pharmazeutische Biochemie und Bioanalytik Publikationen


  • Heimer, P., Tietze, A.A., Bäuml, C.A., Resemann, A., Mayer, F.J., Suckau, D., Ohlenschläger, O., Tietze, D., Imhof, D. (2018) Conformational µ-conotoxin PIIIA isomers revisited: The impact of cysteine pairing on disulfide bond assignment and structure elucidation.   Anal. Chem., doi:10.1021/acs.analchem.7b04854
  • Kumar, A., Wißbrock, A., Goradia, N., Bellstedt, P., Ramachandran, R., Imhof, D., Ohlenschläger, O. (2018) Heme interaction of the intrinsically disordered N-terminal peptide segment of human cystathionine-β-synthase.   Sci Rep, 8. doi:10.1038/s41598-018-20841-z
  • Göder, A., Emmerich, C., Nikolova, T., Kiweler, N., Schreiber M., Kühl, T., Imhof, D., Christmann, M., Heinzel,T., Schneider, G., Krämer, O. (2018) HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130.   Nat Comm., Accepted
  • Grundmann, M., Merten, N., Malfacini, D., Inoue, A., Preis, P., Simon, K., Rüttiger, N., Ziegler, N., Benkel, T., Schmitt, N.K., Ishida, S., Müller, I., Reher, R., Kawakami, K., Inoue, A., Rick, U., Kühl, T., Imhof, D., Aoki, J., König, G.M., Hoffmann, C., Gomeza, J., Wess, J., Kostenis, E. (2018) Lack of beta-arrestin signaling in the absence of active G proteins.   Nat Comm., 9(1):341. doi:10.1038/s41467-017-02661-3






  • Schrage, R., Schmitz, A. L., Gaffal, E., Annala, S., Kehraus, S., Wenzel, D., Büllesbach, K. M., Bald, T., Inoue, A., Shinjo, Y., Galandrin, S., Shridhar, N., Hesse, M., Grundmann, M., Merten, N., Charpentier, T. H., Martz, M., Butcher, A. J., Slodczyk, T., Armando, S., Effern, M., Namkung, Y., Jenkins, L., Horn, V., Stößel, A., Dargatz, H., Tietze, D., Imhof, D., Gales, C., Drewke, C., Müller, C. E., Hölzel, M., Milligan, G., Tobin, A. B., Gomeza, J., Dohlman, H. G., Sondek, J., Harden, T. K., Bouvier, M., Laporte, S. A., Aoki, J., Fleischmann, B. K., Mohr, K., König, G. M., Tüting, T., Kostenis, E. (2015) The experimental power of FR900359 to study Gq-regulated biological processes. Nat. Commun., 6, 10156.
  • Brewitz, H. H., Kühl, T., Goradia, N., Galler, K., Popp, J., Neugebauer, U., Ohlenschläger, O., Imhof, D. (2015) Role of the Chemical Environment beyond the Coordination Site: Structural Insight into Fe III Protoporphyrin Binding to Cysteine-Based Heme-Regulatory Protein Motifs. ChemBioChem, 16, 2216–24.
  • Schubert, E.; Florin, N., Duthie, F., Brewitz, H. H., Kühl, T., Imhof, D., Hagelueken, G., Schiemann, O. (2015) Spectroscopic Studies on Peptides and Proteins with Cysteine-Containing Heme Regulatory Motifs (HRM)  J. Inorg. Biochem., 148, 49–56.
  • Chen, M., Heimer, P., Imhof, D. (2015) Synthetic strategies for polypeptides and proteins by chemical ligation. Amino Acids, 47, 1283-99.


  • Böhm, M., Bäuml, C. A., Hardes, K., Steinmetzer, T., Roeser, D., Schaub, Y., Than, M. E., Biswas, A., Imhof, D. (2014) Novel insights into structure and function of factor XIIIa-inhibitor tridegin. J. Med. Chem., 57, 10355-65.
  • Heimer, P., Tietze, A.A., Böhm, M., Giernoth, R., Kuchenbuch, A., Stark, A., Leipold, E., Heinemann, S.H., Kandt, C., Imhof, D. (2014) Application of Room-Temperature Aprotic and Protic Ionic Liquids for Oxidative Folding of Cysteine-Rich Peptides. ChemBioChem, 15, 2754-65.
  • Seidel, R.A., Schowtka, B., Klopfleisch, M., Kühl, T., Weiland, A., Koch, A., Görls, H., Imhof, D., Pohnert, G., Westerhausen, M. (2014) Total synthesis and characterization of the bilirubin oxidation product (Z)-2-(4-ethenyl-3-methyl-5-oxo-1,5-dihydro-2H-pyrrol-2-ylidene)ethanamide (Z-BOX B). Tetrahedron Lett., 55, 6526-29.
  • Schmitz, A.-L., Schrage, R., Gaffal, E., Charpentier, T. H., Wiest, J., Hiltensperger, G., Morschel, J., Hennen, S., Häußler, D., Horn, V., Wenzel, D., Grundmann, M., Büllesbach, K. M., Schröder, R., Brewitz, H. H., Schmidt, J., Gomeza, J., Galés, C., Fleischmann, B. K., Tüting, T., Imhof, D., Tietze, D., Gütschow, M., Holzgrabe, U., Sondek, J., Harden, T. K., Mohr, K., Kostenis, K. (2014) A cell-permeable inhibitor to trap Gαq proteins in the empty pocket conformation. Chem. Biol., 21, 890-902.
  • Kühl, T., Imhof, D. (2014) Regulatory Fe(II/III) heme: the reconstruction of a molecule's biography. ChemBioChem, 15, 2024-35.
  • Kühl, T., Chen, M., Teichmann, K., Stark, A., Imhof, D. (2014) Ionic liquid 1-ethyl-3-methylimidazolium acetate: an attractive solvent for native chemical ligation of peptides. Tetrahedron Lett., 55, 3658-62.
  • Hennig, D., Schubert, S., Dargatz, H., Kostenis, E., Fahr, A., Schubert, U., Heinzel, T., Imhof, D.(2014) Novel Insights Into Appropriate Encapsulation Methods for Bioactive Compounds Into Polymers: A Study With Peptides and HDAC Inhibitors. Macromol Biosci., 14, 69-80.



  • Böhm, M., Tietze, A. A., Heimer, P., Chen, M., Imhof, D. (2013) How conformation matters: Ionic liquids as reaction media for oxidative folding and native chemical ligation of cysteine-containing peptides. J. Mol. Liq., 192, 67–70.
  • Tietze A.A., Bordusa F., Giernoth R., Imhof, D., Tomas, L., Maaß, A., Mrestani-Klaus, C., Neundorf, I., Oum, K., Reith, D., Stark, A. (2013) On the Nature of Interactions between Ionic Liquids and Small Amino-Acid-Based Biomolecules. ChemPhysChem, 14, 4044–64.
  • Kühl, T., Wißbrock, A., Goradia, N., Sahoo, N., Galler, K., Neugebauer, U., Popp, J., Heinemann, S.H., Ohlenschläger, O., Imhof, D.(2013) Analysis of Fe(III) heme binding to cysteine-containing heme regulatory motifs in proteins, ACS Chem. Biol., 8, 1785-1793.



  • Tietze, A.A., Tietze, D., Ohlenschläger, O., Leipold, E., Ullrich, F., Kühl, T., Mischo, A., Buntkowsky, G., Görlach, M., Heinemann, S.H., Imhof, D. (2012)  Structurally Diverse μ-Conotoxin PIIIA Isomers Block Sodium Channel NaV1.4., Angew. Chem. Int. Ed., 51, 4058-4061.
  • Tietze, A.A., Heimer, P., Stark, A., Imhof, D. (2012) Ionic Liquid Applications in Peptide Chemistry: Synthesis, Purification and Analytical Characterization Processes. Molecules, 17, 4158-4185.
  • Böhm, M., Kühl, T., Hardes, K., Coch, R., Arkona, C., Schlott, B., Steinmetzer, T. and Imhof, D. (2012) Synthesis and Functional Characterization of Tridegin and Its Analogues: Inhibitors and Substrates of Factor XIIIa, ChemMedChem, 7(2), 326-333



  • Kühl, T., Sahoo, N., Nikolajski, M., Schlott, B., Heinemann, S. H. and Imhof, D. (2011), Determination of Hemin-Binding Characteristics of Proteins by a Combinatorial Peptide Library Approach. ChemBioChem, 12(18), 2846-2855
  • Leipold E, Markgraf R, Miloslavina A, Kijas M, Schirmeyer J, Imhof D, Heinemann SH. (2011) Molecular determinants for the subtype specificity of -conotoxin SIIIA targeting neuronal voltage-gated sodium channels. Neuropharmacology 61, 105-111
  • Tietze D, Voigt S, Mollenhauer D, Tischler M, Imhof D, Gutmann T, González L, Ohlenschläger O, Breitzke H, Görlach M, Buntkowsky G. (2011) Revealing the position of the substrate in nickel superoxide dismutase: a model study. Angew Chem Int Ed Engl 50(13), 2946-50



  • Koehn S, Schaefer HW, Ludwig M, Haag N, Schubert US, Seyfarth L, Imhof D, Markert UR, Poehlmann TG. (2010) Cell-specific RNA interference by peptide-inhibited-peptidase-activated siRNAs. J RNAi Gene Silencing 6(2), 422-430
  • Tietze D, Tischler M, Voigt S, Imhof D, Ohlenschläger O, Görlach M, Buntkowsky G. (2010) Development of a functional cis-prolyl bond biomimetic and mechanistic implications for nickel superoxide dismutase. Chemistry 16(25), 7572-7578
  • Valkova C, Mertens C, Weisheit S, Imhof D, Liebmann C. (2010) Activation by Tyrosine Phosphorylation as a Prerequisite for Protein Kinase C{zeta} to Mediate Epidermal Growth Factor Receptor Signaling to ERK. Mol Cancer Research. 8(5), 783-797
  • Miloslavina A, Ebert C, Tietze D, Ohlenschläger O, Englert C, Görlach M, Imhof D. (2010) An unusual peptide from Conus villepinii: Synthesis, solution structure, and cardioactivity. Peptides 31(7), 1292-1300
  • Teichmann K, Kühl T, Könnig I, Wieligmann K, Zacharias M, Imhof D. (2010) Modulation of SHP-1 phosphatase activity by monovalent and bivalent SH2 phosphopeptide ligands. Biopolymers 93, 102-112
  • Teichmann K, Niksch T, Wieligmann K, Zacharias M, Imhof D. (2010) Synthetic Strategies to a Backbone-side Chain Cyclic SHP-1 N-SH2 Ligand Containing N-Functionalized Alkyl Phosphotyrosine. Protein Pept Lett, 17(7), 809-816
  • Miloslavina, A., Leipold, E., Kijas, M., Stark, A., Heinemann, S.H., Imhof, D., A room temperature ionic liquid as convenient solvent for the oxidative folding of conopeptides, J. Peptide Sci., 2009, 15, 72-77.
  • Dubs, M., Weisser, J., Linke, R., Pfuch, A., Imhof, D., Schnabelrauch, M, Dextran-based coating systems for the immobilization of cell adhesion promoting molecules on titanium surfaces, Material Science and Engineering Technology 2009, 40, 853-860.
  • Tietze, D., Breitzke, H., Imhof, D., Buntkowsky, G., New insight into mode of action of NiSOD by investigating metallopeptide substrate models, Chemistry, 2009, 15, 517-523.
  • Fan, Y., Ludwig, R., Imhof, D., Scriba, G.E., Development of a capillary electrophoresis based assay of sirtuin enzymes, Electrophoresis 2008, 29, 3717-3723.
  • Musharraf, A., Markschies, N., Teichmann, K., Pankratz, S., Landgraf, K., Englert, C., Imhof, D., Eyes absent proteins: characterization of substrate specificity and phosphatase activity of mutants associated with branchial, otic and renal anomalies, ChemBioChem 2008, 9, 2285-2294.
  • Valkova, C., Maerz, S., Imhof, D., Liebmann, C., Protein kinase C{epsilon} may act as EGF-inducible scaffold protein for phospholipase C{gamma}1. Cell. Signal. 2007, 19, 1830-1843.
  • Teichmann, K., Winkler, R., Hampel, K., Trümpler, A., Böhmer, F. D., Imhof, D., Monitoring phosphatase reactions of multiple phosphorylated substrates by reversed-phase HPLC. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2007, 853, 204-213.
  • Hampel, K., Kaufhold, I., Zacharias, M., Böhmer, F. D., Imhof, D., Phosphopeptide ligands of the SHP-1 N-SH2 domain: Effects on binding and stimulation of phosphatase activity. ChemMedChem 2006, 1, 869-877.
  • Imhof, D., Wavreille, A.-S., May, A., Zacharias, M., Tridandapani, S., Pei, D., Sequence specificity of SHP-1 and SHP-2 Src Homology 2 domains: Critical roles of residues beyond the pY+3 position. J. Biol. Chem. 2006, 281, 20271-20282.
  • Imhof, D., Wieligmann, K., Hampel, K., Nothmann, D., Zoda, M. S., Schmidt-Arras, D., Zacharias, M., Reissmann, S., Böhmer, F.D., Design and biological evaluation of linear and cyclic phosphopeptide ligands of the N-terminal SH2 domain of protein tyrosine phosphatase SHP-1. J. Med. Chem. 2005, 48, 1528-1539.
  • Imhof, D., Nothmann, D., Zoda, M. S., Hampel, K., Wegner, J., Böhmer, F. D., and Reissmann, S., Synthesis of linear and cyclic phosphopeptides as ligands for the N-terminal SH2 domain of protein tyrosine phosphatase SHP-1, J. Pept. Sci. 2005, 11, 390-400.