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Prof. Dr. Diana Imhof

Universität Bonn

Pharmazeutisches Institut

Pharmazeutische Biochemie und Bioanalytik

An der Immenburg 4

D-53121 Bonn

 

Telefon: 0228 73 5254
Fax: 0228 73 6829
E-Mail: dimhof [at] uni-bonn.de

 

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Pharmazeutische Biochemie und Bioanalytik
 
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Publikationen

2018
  • Paul George, A.A., Heimer, P., Maaß, A., Hamaekers, J., Hofmann-Apitius, M., Biswas, A., Imhof, D. (2018) Insights into the Folding of Disulfide-Rich μ-Conotoxins. ACS Omega, 3(10), 12330-12340
  • Heimer, P., Schmitz, T., Bäuml, C. A., Imhof, D. (2018) Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities. J. Vis. Exp. (140), e58368
  • Peherstorfer, S., Brewitz, H. H., Paul George, A.A., Wißbrock, A., Adam, J.M., Schmitt, L., Imhof, D. (2018) Insights into mechanism and functional consequences of heme binding to hemolysin-activating lysine acyltransferase HlyC from Escherichia coli. Biochim. Biophys. Acta - General Subjects, 1862(9), 1964-1972
  • Reher, R., Kühl, T., Annala, S., Benkel, T., Kaufmann, D., Nubbemeyer, B., Odhiambo, J.P., Heimer, P., Bäuml, C.A., Kehraus, S., Crüsemann, M., Kostenis, E., Tietze, D., König, G.M., Imhof, D. (2018) Deciphering specificity determinants for FR900359‐derived Gαq inhibitors based on computational and structure‐activity studies. ChemMedChem, 13(16), 1634-1643
  • Oppermann, S., Oppermann, C., Böhm, M., Kühl, T., Imhof, D., Kragl, U. (2018) Quantification of amino acids and peptides in an ionic liquid based aqueous two-phase system by LC–MS analysis. AMB Express, 8:66
  • Lichtenberg, N., Menges, R., Ageev, V., Paul George, A.A., Heimer, P., Imhof, D., Lawonn, K. (2018) Analyzing residue surface proximity to interpret molecular dynamics. Comput Graph Forum, 37(3), 379-390
  • Heimer, P., Tietze, A.A., Bäuml, C.A., Resemann, A., Mayer, F.J., Suckau, D., Ohlenschläger, O., Tietze, D., Imhof, D. (2018) Conformational µ-conotoxin PIIIA isomers revisited: The impact of cysteine pairing on disulfide bond assignment and structure elucidation. Anal. Chem., 90(5), 3321-3327
  • Kumar, A., Wißbrock, A., Goradia, N., Bellstedt, P., Ramachandran, R., Imhof, D., Ohlenschläger, O. (2018) Heme interaction of the intrinsically disordered N-terminal peptide segment of human cystathionine-β-synthase. Sci Rep, 8:2474
  • Göder, A., Emmerich, C., Nikolova, T., Kiweler, N., Schreiber M., Kühl, T., Imhof, D., Christmann, M., Heinzel,T., Schneider, G., Krämer, O. (2018) HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130. Nat Comm., 9:764
  • Grundmann, M., Merten, N., Malfacini, D., Inoue, A., Preis, P., Simon, K., Rüttiger, N., Ziegler, N., Benkel, T., Schmitt, N.K., Ishida, S., Müller, I., Reher, R., Kawakami, K., Inoue, A., Rick, U., Kühl, T., Imhof, D., Aoki, J., König, G.M., Hoffmann, C., Gomeza, J., Wess, J., Kostenis, E. (2018) Lack of beta-arrestin signaling in the absence of active G proteins. Nat Comm., 9(1):341

 

2017 (Umzug in neue Labore und Etablierung der "Protein Synthesis & Bioanalytics Core Facility")
  • Tietze, D., Sartorius, J., Koley Seth, B., Herr, K., Heimer, P., Imhof, D., Mollenhauer, D., Buntkowsky, G. (2017) New insights into the mechanism of nickel superoxide degradation from studies of model peptides. Sci Rep, 7:17194
  • Gessner, G., Sahoo, N., Swain, S.M., Hirth, G., Schönherr, R., Mede, R., Westerhausen, M., Brewitz, H.H., Heimer, P., Imhof, D., Hoshi, T., Heinemann, S.H. (2017) CO-independent modification of K+ channels by tricarbonyldichlororuthenium(II) dimer (CORM-2). Eur J Pharmacol, 815(15), 33-41
  • Wißbrock, A., Imhof D. (2017) A tough nut to crack: Intracellular detection and quantification of heme in malaria parasites by a genetically encoded protein sensor. ChemBioChem, 18(16), 1561-1564
  • Wißbrock, A., Kühl, T., Silbermann, K., Becker, A. J., Ohlenschläger, O., Imhof, D. (2017) Synthesis and evaluation of Abeta-derived and Abeta-independent enhancers of the peroxidase-like activity of heme. J. Med. Chem., 60(1), 373-385

 

2016
  • Brewitz, H. H., Hagelüken, G., Imhof, D. (2016) Structural and functional diversity of transient heme binding to bacterial proteins. Biochim. Biophys. Acta - General Subjects, 1861(3), 683-697
  • Leipold, E., Ullrich, F., Thiele, M., Tietze, A. A., Terlau, H., Imhof, D., Heinemann, S. H. (2016) Subtype-specific block of voltage-gated K+ channels by μ-conopeptides. Biochem. Biophys. Res. Commun., 482(4), 1135-1140
  • Hennig, D., Imhof, D. (2016) Encapsulation of the HDACi Ex527 into liposomes and polymer-based particles. In: Assessment of HDAC Functions and Development of their Inhibitors: Methods and Protocols, (Springer, Ed. O. Krämer). Methods in Molecular Biology book series (MIMB), 1510, 387-398
  • Goradia, N., Wißbrock, A., Wiedemann, C., Bordusa, F., Ramachandran, R., Imhof, D., Ohlenschläger, O. (2016) 1H, 13C, and 15N resonance assignments for the pro-inflammatory cytokine interleukin-36α. Biomolecular NMR assignments, 10(2), 329-333
  • Blaurock, N., Schmerler, D., Hünniger, K., Kurzaj, O., Ludewig, K., Baier, M., Brunkhorst, F. M., Imhof, D., Kiehntopf, M. (2016) C-Terminal Alpha-1 Antitrypsin Peptide: A New Sepsis Biomarker with Immunomodulatory Function. Mediators in Inflammation, 2016:6129437
  • Tietze, D., Leipold, E., Heimer, P., Böhm, M., Winschel, W., Imhof, D., Heinemann, S. H., Tietze, A. A. (2016) Molecular interaction of δ-conopeptide EVIA with voltage-gated Na+ channels. Biochim. Biophys. Acta - General Subjects, 1860(9), 2053-2063
  • Hagelueken, G., Hofmann, J., Schubert, E., Duthie, F. G., Florin, N., Konrad, L., Imhof, D., Morgner, N., Schiemann, O. (2016) Studies on the crystal- and solution structure of FeoB from Escherichia coli BL21 using X-ray crystallography, PELDOR spectroscopy and mass spectrometry. Biophys. J., 110(12), 2642-2650
  • Brewitz, H. H., Goradia, N., Schubert, E., Galler, K., Kühl, T., Syllwasschy, B., Popp, J., Neugebauer, U., Hagelueken, G., Schiemann, O., Ohlenschläger, O., Imhof, D. (2016) Heme interacts with histidine- and tyrosine-based protein motifs and inhibits enzymatic activity of chloramphenicol acetyltransferase from Escherichia coliBiochim. Biophys. Acta - General Subjects, 1860(9), 1343-1353
  • Coburger, I., Schaub, Y., Roeser, D., Hardes, K., Maeder, P., Klee, N., Steinmetzer, T., Imhof, D., Diederich, W. E., Than, M. E. (2016) Identification of inhibitors of the transmembrane protease FlaK of Methanococcus maripaludis. Microbiologyopen, 5(4), 637-646
  • Grizić, D., Heimer P., Vranić, E., Imhof, D., Lamprecht, A. (2016) From propylene carbonate to 3,5-diacetyl-1,4-dihydro-2,6-lutidine: a novel quantification approach. Talanta, 151, 75-82

 

2015
  • Schrage, R., Schmitz, A. L., Gaffal, E., Annala, S., Kehraus, S., Wenzel, D., Büllesbach, K. M., Bald, T., Inoue, A., Shinjo, Y., Galandrin, S., Shridhar, N., Hesse, M., Grundmann, M., Merten, N., Charpentier, T. H., Martz, M., Butcher, A. J., Slodczyk, T., Armando, S., Effern, M., Namkung, Y., Jenkins, L., Horn, V., Stößel, A., Dargatz, H., Tietze, D., Imhof, D., Gales, C., Drewke, C., Müller, C. E., Hölzel, M., Milligan, G., Tobin, A. B., Gomeza, J., Dohlman, H. G., Sondek, J., Harden, T. K., Bouvier, M., Laporte, S. A., Aoki, J., Fleischmann, B. K., Mohr, K., König, G. M., Tüting, T., Kostenis, E. (2015) The experimental power of FR900359 to study Gq-regulated biological processes. Nat. Commun., 6:10156
  • Brewitz, H. H., Kühl, T., Goradia, N., Galler, K., Popp, J., Neugebauer, U., Ohlenschläger, O., Imhof, D. (2015) Role of the Chemical Environment beyond the Coordination Site: Structural Insight into Fe III Protoporphyrin Binding to Cysteine-Based Heme-Regulatory Protein Motifs. ChemBioChem, 16(15), 2216–24
  • Schubert, E.; Florin, N., Duthie, F., Brewitz, H. H., Kühl, T., Imhof, D., Hagelueken, G., Schiemann, O. (2015) Spectroscopic Studies on Peptides and Proteins with Cysteine-Containing Heme Regulatory Motifs (HRM). J. Inorg. Biochem., 148, 49–56
  • Chen, M., Heimer, P., Imhof, D. (2015) Synthetic strategies for polypeptides and proteins by chemical ligation. Amino Acids, 47(7), 1283-1299

 

2014
  • Böhm, M., Bäuml, C. A., Hardes, K., Steinmetzer, T., Roeser, D., Schaub, Y., Than, M. E., Biswas, A., Imhof, D. (2014) Novel insights into structure and function of factor XIIIa-inhibitor tridegin. J. Med. Chem., 57(24), 10355-10365
  • Heimer, P., Tietze, A.A., Böhm, M., Giernoth, R., Kuchenbuch, A., Stark, A., Leipold, E., Heinemann, S.H., Kandt, C., Imhof, D. (2014) Application of Room-Temperature Aprotic and Protic Ionic Liquids for Oxidative Folding of Cysteine-Rich Peptides. ChemBioChem, 15(18), 2754-2765
  • Seidel, R.A., Schowtka, B., Klopfleisch, M., Kühl, T., Weiland, A., Koch, A., Görls, H., Imhof, D., Pohnert, G., Westerhausen, M. (2014) Total synthesis and characterization of the bilirubin oxidation product (Z)-2-(4-ethenyl-3-methyl-5-oxo-1,5-dihydro-2H-pyrrol-2-ylidene)ethanamide (Z-BOX B). Tetrahedron Lett., 55(48), 6526-6529
  • Schmitz, A.-L., Schrage, R., Gaffal, E., Charpentier, T. H., Wiest, J., Hiltensperger, G., Morschel, J., Hennen, S., Häußler, D., Horn, V., Wenzel, D., Grundmann, M., Büllesbach, K. M., Schröder, R., Brewitz, H. H., Schmidt, J., Gomeza, J., Galés, C., Fleischmann, B. K., Tüting, T., Imhof, D., Tietze, D., Gütschow, M., Holzgrabe, U., Sondek, J., Harden, T. K., Mohr, K., Kostenis, K. (2014) A cell-permeable inhibitor to trap Gαq proteins in the empty pocket conformation. Chem. Biol., 21(7), 890-902
  • Kühl, T., Imhof, D. (2014) Regulatory Fe(II/III) heme: the reconstruction of a molecule's biography. ChemBioChem, 15(14), 2024-2035.
  • Kühl, T., Chen, M., Teichmann, K., Stark, A., Imhof, D. (2014) Ionic liquid 1-ethyl-3-methylimidazolium acetate: an attractive solvent for native chemical ligation of peptides. Tetrahedron Lett., 55(27), 3658-3662
  • Hennig, D., Schubert, S., Dargatz, H., Kostenis, E., Fahr, A., Schubert, U., Heinzel, T., Imhof, D.(2014) Novel Insights Into Appropriate Encapsulation Methods for Bioactive Compounds Into Polymers: A Study With Peptides and HDAC Inhibitors. Macromol Biosci., 14(1), 69-80

 

2013

  • Böhm, M., Tietze, A. A., Heimer, P., Chen, M., Imhof, D. (2013) How conformation matters: Ionic liquids as reaction media for oxidative folding and native chemical ligation of cysteine-containing peptides. J. Mol. Liq., 192, 67–70
  • Tietze A.A., Bordusa F., Giernoth R., Imhof, D., Tomas, L., Maaß, A., Mrestani-Klaus, C., Neundorf, I., Oum, K., Reith, D., Stark, A. (2013) On the Nature of Interactions between Ionic Liquids and Small Amino-Acid-Based Biomolecules. ChemPhysChem, 14(18), 4044–4064
  • Kühl, T., Wißbrock, A., Goradia, N., Sahoo, N., Galler, K., Neugebauer, U., Popp, J., Heinemann, S.H., Ohlenschläger, O., Imhof, D.(2013) Analysis of Fe(III) heme binding to cysteine-containing heme regulatory motifs in proteins, ACS Chem. Biol., 8(8), 1785-1793

 

2012

  • Tietze, A.A., Tietze, D., Ohlenschläger, O., Leipold, E., Ullrich, F., Kühl, T., Mischo, A., Buntkowsky, G., Görlach, M., Heinemann, S.H., Imhof, D. (2012)  Structurally Diverse μ-Conotoxin PIIIA Isomers Block Sodium Channel NaV1.4. Angew. Chem. Int. Ed., 51(17), 4058-4061
  • Tietze, A.A., Heimer, P., Stark, A., Imhof, D. (2012) Ionic Liquid Applications in Peptide Chemistry: Synthesis, Purification and Analytical Characterization Processes. Molecules, 17(4), 4158-4185
  • Böhm, M., Kühl, T., Hardes, K., Coch, R., Arkona, C., Schlott, B., Steinmetzer, T. and Imhof, D. (2012) Synthesis and Functional Characterization of Tridegin and Its Analogues: Inhibitors and Substrates of Factor XIIIa. ChemMedChem, 7(2), 326-333

 

2011

  • Kühl, T., Sahoo, N., Nikolajski, M., Schlott, B., Heinemann, S. H. and Imhof, D. (2011), Determination of Hemin-Binding Characteristics of Proteins by a Combinatorial Peptide Library Approach. ChemBioChem, 12(18), 2846-2855
  • Leipold E, Markgraf R, Miloslavina A, Kijas M, Schirmeyer J, Imhof D, Heinemann SH. (2011) Molecular determinants for the subtype specificity of -conotoxin SIIIA targeting neuronal voltage-gated sodium channels. Neuropharmacology 61, 105-111
  • Tietze D, Voigt S, Mollenhauer D, Tischler M, Imhof D, Gutmann T, González L, Ohlenschläger O, Breitzke H, Görlach M, Buntkowsky G. (2011) Revealing the position of the substrate in nickel superoxide dismutase: a model study. Angew Chem Int Ed Engl,  50(13), 2946-2950

 

2005-2010

  • Koehn S, Schaefer HW, Ludwig M, Haag N, Schubert US, Seyfarth L, Imhof D, Markert UR, Poehlmann TG. (2010) Cell-specific RNA interference by peptide-inhibited-peptidase-activated siRNAs. J RNAi Gene Silencing 6(2), 422-430
  • Tietze D, Tischler M, Voigt S, Imhof D, Ohlenschläger O, Görlach M, Buntkowsky G. (2010) Development of a functional cis-prolyl bond biomimetic and mechanistic implications for nickel superoxide dismutase. Chemistry 16(25), 7572-7578
  • Valkova C, Mertens C, Weisheit S, Imhof D, Liebmann C. (2010) Activation by Tyrosine Phosphorylation as a Prerequisite for Protein Kinase Cζ to Mediate Epidermal Growth Factor Receptor Signaling to ERK. Mol Cancer Research. 8(5), 783-797
  • Miloslavina A, Ebert C, Tietze D, Ohlenschläger O, Englert C, Görlach M, Imhof D. (2010) An unusual peptide from Conus villepinii: Synthesis, solution structure, and cardioactivity. Peptides 31(7), 1292-1300
  • Teichmann K, Kühl T, Könnig I, Wieligmann K, Zacharias M, Imhof D. (2010) Modulation of SHP-1 phosphatase activity by monovalent and bivalent SH2 phosphopeptide ligands. Biopolymers 93(1), 102-112
  • Teichmann K, Niksch T, Wieligmann K, Zacharias M, Imhof D. (2010) Synthetic Strategies to a Backbone-side Chain Cyclic SHP-1 N-SH2 Ligand Containing N-Functionalized Alkyl Phosphotyrosine. Protein Pept Lett, 17(7), 809-816
  • Miloslavina, A., Leipold, E., Kijas, M., Stark, A., Heinemann, S.H., Imhof, D., (2009) A room temperature ionic liquid as convenient solvent for the oxidative folding of conopeptides . J. Peptide Sci., 15(2), 72-77
  • Dubs, M., Weisser, J., Linke, R., Pfuch, A., Imhof, D., Schnabelrauch, M. (2009) Dextran-based coating systems for the immobilization of cell adhesion promoting molecules on titanium surfaces. Material Science and Engineering Technology, 40(11), 853-860.
  • Tietze, D., Breitzke, H., Imhof, D., Buntkowsky, G. (2009) New insight into mode of action of NiSOD by investigating metallopeptide substrate models. Chemistry, 15(2), 517-523
  • Fan, Y., Ludwig, R., Imhof, D., Scriba, G.E. (2008) Development of a capillary electrophoresis based assay of sirtuin enzymes. Electrophoresis, 29(18), 3717-3723
  • Musharraf, A., Markschies, N., Teichmann, K., Pankratz, S., Landgraf, K., Englert, C., Imhof, D. (2008) Eyes absent proteins: characterization of substrate specificity and phosphatase activity of mutants associated with branchial, otic and renal anomalies. ChemBioChem, 9(14), 2285-2294
  • Valkova, C., Maerz, S., Imhof, D., Liebmann, C. (2007) Protein kinase Cε may act as EGF-inducible scaffold protein for phospholipase Cγ1. Cell. Signal., 19(9), 1830-1843 
  • Teichmann, K., Winkler, R., Hampel, K., Trümpler, A., Böhmer, F. D., Imhof, D. (2007) Monitoring phosphatase reactions of multiple phosphorylated substrates by reversed-phase HPLC. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 853(1-2) 204-213
  • Hampel, K., Kaufhold, I., Zacharias, M., Böhmer, F. D., Imhof, D. (2006) Phosphopeptide ligands of the SHP-1 N-SH2 domain: Effects on binding and stimulation of phosphatase activity. ChemMedChem, 1(8), 869-877
  • Imhof, D., Wavreille, A.-S., May, A., Zacharias, M., Tridandapani, S., Pei, D. (2006)Sequence specificity of SHP-1 and SHP-2 Src Homology 2 domains: Critical roles of residues beyond the pY+3 position. J. Biol. Chem., 281, 20271-20282.
  • Imhof, D., Wieligmann, K., Hampel, K., Nothmann, D., Zoda, M. S., Schmidt-Arras, D., Zacharias, M., Reissmann, S., Böhmer, F.D. (2005) Design and biological evaluation of linear and cyclic phosphopeptide ligands of the N-terminal SH2 domain of protein tyrosine phosphatase SHP-1. J. Med. Chem., 48(5), 1528-1539 
  • Imhof, D., Nothmann, D., Zoda, M. S., Hampel, K., Wegner, J., Böhmer, F. D., and Reissmann, S. (2005) Synthesis of linear and cyclic phosphopeptides as ligands for the N-terminal SH2 domain of protein tyrosine phosphatase SHP-1, J. Pept. Sci., 11(7), 390-400
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